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HCG

FDA Approved

Human Chorionic Gonadotropin | LH Receptor Agonist

Dose 250-1500 IU (lower for TRT adjunct, higher for fertility)
Frequency 2-3 times weekly, or every other day for lower doses
Cycle Ongoing with TRT or 3-6 months for fertility protocols
Storage Lyophilized: Room temperature. Reconstituted: 2-8°C, use within 30-60 days

HCG is a glycoprotein hormone naturally produced by the placenta during pregnancy that binds LH receptors to stimulate testosterone and estrogen biosynthesis. FDA-approved for cryptorchidism, hypogonadotropic hypogonadism, and ovulation induction.

Mechanism of Action

Binds to LH receptors on Leydig cells in testes, stimulating testosterone production with a half-life of 24-36 hours, peak levels 6-12 hours post-injection, and 40-50% bioavailability via SubQ or IM routes.

Key Benefits

  • Maintains testicular function during TRT
  • Preserves fertility and prevents testicular atrophy
  • Stimulates endogenous testosterone production
  • Induces ovulation in women
  • FDA-approved for multiple indications
  • Well-established safety profile
Molecular Weight
36,700 Da
Chain Length
237 amino acids (alpha: 92, beta: 145)
Type
Heterodimeric glycoprotein

Male Fertility

  • TRT Adjunct

    Maintains intratesticular testosterone at baseline during testosterone therapy, preventing atrophy and preserving fertility.

  • Hypogonadotropic Hypogonadism

    FDA-approved for secondary hypogonadism; combined with FSH for spermatogenesis induction.

  • Post-Cycle Therapy

    Restores testicular function after anabolic steroid cycles.

Female Fertility

  • Ovulation Induction

    FDA-approved trigger for follicular maturation; 15-25% pregnancy rate per cycle.

Pediatric

  • Cryptorchidism

    FDA-approved for prepubertal undescended testes not due to anatomical obstruction; ~25% success rate.

Subcutaneous or intramuscular injection. Administer 2-3 times weekly, evenly spaced. For TRT, many inject HCG on days between testosterone injections.

GoalDoseFrequencyRoute
TRT Adjunct (Low)250-500 IUEvery other daySubQ/IM
TRT Adjunct (Standard)500-1000 IUTwice weeklySubQ/IM
HCG Monotherapy1500-2000 IU2-3x weeklyIM
Fertility (with FSH)1500-2000 IU2-3x weeklyIM
Cryptorchidism (Pediatric)1000-5000 IU2-3x weekly for 3-4 weeksIM
Ovulation Trigger (Female)5000-10,000 IUSingle doseIM/SubQ
PCT Protocol1000-1500 IUEvery other day for 2-3 weeksSubQ/IM

Reconstitution Instructions

Materials Needed:
  • HCG lyophilized powder vial (typically 5000 or 10,000 IU)
  • Bacteriostatic water or sodium chloride diluent (provided)
  • Insulin syringes (29-31 gauge for SubQ)
  • Alcohol prep pads
  1. 1 Remove vial and diluent from packaging
  2. 2 Clean rubber stoppers with alcohol swabs
  3. 3 Draw diluent into syringe (typically 1-2mL provided)
  4. 4 Slowly inject diluent into HCG vial, aiming at vial wall
  5. 5 Gently swirl to dissolve - do not shake vigorously
  6. 6 Allow to sit until completely dissolved and clear
  7. 7 Calculate concentration (e.g., 5000 IU in 2mL = 2500 IU/mL)
  8. 8 Label with reconstitution date and concentration
  9. 9 Store reconstituted HCG at 2-8°C
  10. 10 Use within 30-60 days
Testosterone

Commonly combined in TRT to maintain testicular function and preserve fertility.

synergistic
Clomiphene (Clomid)

Use sequentially; both affect HPG axis.

monitor
Aromatase Inhibitors

Commonly combined; HCG increases intratesticular aromatase activity.

compatible
GnRH Analogs

Monitor combination effects on gonadotropin axis.

monitor
FSH (Follitropin)

Synergistic for fertility; 70-90% spermatogenesis induction rate.

synergistic
Kisspeptin

Complementary mechanisms for HPG axis stimulation.

compatible
Thyroid Hormones

HCG has weak TSH-like activity; monitor thyroid function.

monitor
Metformin

Protective combination for metabolic health.

compatible
Day 1-3

Cellular-level action begins; no immediate noticeable effects

Week 1-2

Testosterone increase detectable on labs; possible mood/energy improvement

Week 2-4

Testicular fullness/size improvement noticeable; improved well-being

Week 4-8

Stable testosterone levels; fertility parameters beginning to improve

Month 2-3

Sperm count improvements if used for fertility; sustained testicular function

Long-term

Maintained testicular size and function with ongoing use

Common Side Effects

  • Gynecomastia (breast tenderness/swelling) due to increased estrogen
  • Headaches, irritability, and mood swings (especially initially)
  • Fluid retention and edema
  • Potential antibody formation with long-term use

Stop Signs - Discontinue if:

  • Signs of gynecomastia (breast tenderness, swelling, nipple sensitivity)
  • Severe or persistent headaches
  • Signs of blood clots (leg swelling/pain, shortness of breath, chest pain)
  • Allergic reactions (rash, hives, difficulty breathing, facial swelling)
  • Severe abdominal pain or bloating in women (possible OHSS)
  • Testicular pain or swelling beyond normal
  • Significant mood changes (depression, aggression, severe irritability)
  • Vision changes

Contraindications

  • Hormone-sensitive cancers (prostate, breast)
  • Pregnancy (except as prescribed)
  • Precocious puberty risk in children

Good Signs

  • White to off-white lyophilized powder or cake in sealed vial
  • Completely clear solution after reconstitution
  • Proper labeling: Pregnyl, Novarel (urinary), Ovidrel (recombinant)
  • Clear expiration and lot number
  • Cold chain compliance (recombinant requires refrigeration throughout)

Warning Signs

  • Generic/compounding pharmacy products - quality varies
  • Ensure compounding pharmacy is accredited

Bad Signs

  • Cloudiness, discoloration, or floating particles indicates degradation
  • Compromised vial seal or expired product
  • HCG for Intratesticular Testosterone Maintenance
    (2005)

    250 IU HCG every other day during TRT maintains intratesticular testosterone at baseline, preventing atrophy and preserving fertility.

  • HCG Monotherapy for Hypogonadism
    (2013)

    HCG monotherapy (1500-2000 IU twice weekly) effectively increased testosterone and improved symptoms while maintaining fertility.

  • Spermatogenesis Induction with HCG/FSH
    (2018)

    Combined HCG and FSH therapy induces spermatogenesis in 70-90% of men with hypogonadotropic hypogonadism.

  • Cryptorchidism Treatment Meta-Analysis (Cochrane)
    (2014)

    Meta-analysis of 1,231 boys showing ~25% success rate for hormonal treatment of cryptorchidism.

  • Ovulation Induction Success Rates
    (2017)

    HCG trigger achieves pregnancy rates of 15-25% per cycle in clomiphene/letrozole protocols.

Disclaimer

This information is for educational and research purposes only. Consult a healthcare professional before use.