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DSIP

Emerging

Delta Sleep-Inducing Peptide | Sleep & Stress Modulator

Dose 100-300mcg
Frequency 1x daily
Cycle 5-10 days
Storage 2-8°C refrigerated

DSIP is a naturally occurring nonapeptide discovered in 1974 in rabbit brain. Originally isolated for sleep-promoting properties, it has diverse neuromodulatory effects including stress reduction, pain modulation, and endocrine regulation without traditional sedative effects.

Mechanism of Action

Modulates multiple neurotransmitter systems including GABA enhancement, NMDA receptor interaction, and endogenous opioid system modulation. Also regulates hypothalamic-pituitary-adrenal axis for stress response.

Key Benefits

  • Enhanced sleep quality through slow-wave sleep promotion
  • Stress reduction via cortisol modulation
  • Potential pain relief
  • Mood stabilization without traditional sedative effects
  • No tolerance or dependence reported
  • Short cycle duration (5-10 days)
Molecular Weight
848.81 Da
Chain Length
9 amino acids
Type
Nonapeptide
Amino Acid Sequence
One-letter: WAGGDASGE
H₂N
W 1
O C
N
A 2
O C
N
H
G 3
O C
N
H
G 4
O C
N
D 5
O C
N
A 6
O C
N
S 7
O C
N
H
G 8
O C
N
E 9
COOH
Trp
1

Tryptophan

Position 1

Ala
2

Alanine

Position 2

Gly
3

Glycine

Position 3

Gly
4

Glycine

Position 4

Asp
5

Aspartic Acid

Position 5

Ala
6

Alanine

Position 6

Ser
7

Serine

Position 7

Gly
8

Glycine

Position 8

Glu
9

Glutamic Acid

Position 9

N-terminus C-terminus
Hydrophobic
Polar
Positive (+)
Negative (-)
Modified

Sleep

  • Deep Sleep Enhancement

    Promotes delta wave sleep, the most restorative sleep phase for physical recovery.

  • Sleep Efficiency

    Reduces sleep latency and nocturnal awakenings without morning grogginess.

  • Natural Sleep Architecture

    Supports natural sleep cycles rather than forcing sedation like traditional sleep aids.

Mood

  • Stress Response Modulation

    Helps regulate cortisol and stress response through HPA axis modulation.

  • Mood Stabilization

    Supports emotional balance without sedation.

  • Anxiety Reduction

    May help reduce anxiety through neuromodulatory effects.

Pain & Recovery

  • Pain Modulation

    Significantly reduced pain levels in 6 of 7 patients with chronic pain conditions.

  • Withdrawal Support

    Helps manage withdrawal symptoms in alcohol and opioid-dependent patients.

  • Athletic Recovery

    Supports post-training recovery through enhanced sleep quality.

Subcutaneous injection is the standard route. Best taken 30-60 minutes before bedtime. Some prefer earlier evening dosing for gradual onset.

GoalDoseFrequencyRoute
Sleep enhancement100-200mcgOnce nightlySubQ
Chronic pain support250-300mcgDailySubQ or IV
Stress management150mcgEveningSubQ
Withdrawal support200-300mcgTwice dailyIV preferred
Athletic recovery100-150mcgPost-trainingSubQ

Reconstitution Instructions

Materials Needed:
  • DSIP peptide vial (1-5mg typical)
  • Bacteriostatic water (1-2mL)
  • Insulin syringes (0.3-0.5mL)
  • Alcohol swabs
  • Sterile needle for mixing
  1. 1 Clean vial tops with alcohol swab and let dry completely
  2. 2 For 1mg vial, add 1mL bacteriostatic water (creates 1000mcg/mL)
  3. 3 Inject water slowly down vial side to prevent foaming
  4. 4 Gently roll between palms - do not shake
  5. 5 Solution should be clear and colorless
  6. 6 For 100mcg dose, draw 0.1mL (10 units on insulin syringe)
  7. 7 Store reconstituted peptide in refrigerator immediately
Melatonin

Both regulate sleep-wake cycles; complementary benefits.

synergistic
GABA Agonists

DSIP enhances GABA - watch for excessive sedation.

monitor
Cortisol Modulators

Can combine for comprehensive stress management.

compatible
Selank

Complementary anxiolytic effects.

synergistic
Semax

Different mechanisms; can optimize 24-hour function.

compatible
Opioid Medications

May modulate opioid receptors; requires professional supervision.

monitor
Growth Hormone Peptides

No direct interaction; DSIP may support natural GH release during sleep.

compatible
Epitalon

Both support circadian regulation and sleep quality.

synergistic
First Dose

Noticeable sleep pressure and easier sleep onset

Night 1-3

Deeper sleep, fewer awakenings, vivid dreams possible

Day 2-5

Improved morning refreshment, stable daytime energy

Day 5-10

Cumulative stress reduction, mood improvements

Post-Cycle

Benefits may persist 1-2 weeks after stopping

Common Side Effects

  • Generally well-tolerated with minimal side effects
  • Mild drowsiness or dizziness initially (some users)
  • Occasional headaches in sensitive individuals
  • No tolerance or dependence reported in studies

Stop Signs - Discontinue if:

  • Excessive daytime sedation
  • Paradoxical insomnia or agitation
  • Persistent headaches
  • Any allergic reactions
  • Mood changes or depression
  • Unusual dreams causing distress

Contraindications

  • Avoid driving until effects are known
  • Pregnancy or breastfeeding

Good Signs

  • White lyophilized powder before reconstitution
  • Clear and colorless solution when properly mixed
  • Reputable source with third-party testing

Warning Signs

  • Short stability period - use within 14 days when reconstituted
  • Protect from light exposure and temperature fluctuations
  • Limited nasal formulation stability (use within 10 days)

Bad Signs

  • Yellow or cloudy solution indicates degradation or contamination
  • Discolored powder
  • Chronic Insomnia Double-Blind Study
    (1991)

    15-30 nmol for 6 consecutive nights improved objective sleep quality with higher sleep efficiency and shorter sleep latency compared to placebo.

  • Chronic Pain Pilot Study
    (1984)

    25 nmol IV for 10 days significantly reduced pain levels in 6 of 7 patients with chronic pain conditions including migraine and tinnitus.

  • Alcohol and Opioid Withdrawal Study
    (1984)

    DSIP administration helped manage withdrawal symptoms in both alcohol and opioid-dependent patients over 5-10 days.

  • Sleep Pressure Induction Study
    (1983)

    Single 25 nmol/kg IV dose showed 59% increase in total sleep time within 130 minutes; improved subsequent night sleep efficiency.

  • Stroke Recovery in Rats
    (2021)

    120mcg/kg nasal for 7 days enhanced motor recovery without significantly reducing infarct size.

Disclaimer

This information is for educational and research purposes only. Consult a healthcare professional before use.