Wolverine Stack
BPC-157 + TB-500 | Tissue Repair & Recovery Protocol
Combines BPC-157 and TB-500 for synergistic tissue repair and recovery. 87.5% improvement in knee pain patients per Lee & Padgett 2021 study.
Mechanism of Action
BPC-157 increases actin production and modulates nitric oxide for vascular effects; TB-500 sequesters actin for cell migration; synergistic enhancement of fibroblast and immune cell movement to injury sites.
Key Benefits
- Complementary healing mechanisms
- 87.5% improvement in knee pain patients
- Reduced inflammation and enhanced cell migration
- Promotes angiogenesis and reduces scarring
N/A Complex or non-standard sequence format
Tissue Repair
- Tendon & Ligament Healing
Both peptides show evidence for accelerated repair; BPC-157 improved Achilles tendon healing in animal models.
- Muscle Injury Recovery
BPC-157 demonstrates enhanced muscle regeneration; TB-500 promotes cell migration essential for repair.
- Joint Pain & Cartilage
Lee & Padgett study showed 87.5% of knee pain patients improved with BPC-157/TB4 combination.
Wound Healing
- Accelerated Wound Closure
TB-500 Phase 2 trials showed approximately one month faster healing in ulcer patients.
Anti-Inflammatory
- Inflammatory Cytokine Reduction
Both peptides modulate inflammatory pathways through different mechanisms.
Subcutaneous injection recommended; most studied and effective route.
| Goal | Dose | Frequency | Route |
|---|---|---|---|
| General Recovery Protocol | BPC-157: 250mcg 2x/day + TB-500: 2mg 2x/week | BPC-157 daily, TB-500 twice weekly | SubQ |
| Intensive Injury Recovery | BPC-157: 500mcg 2x/day + TB-500: 2.5mg 2x/week | BPC-157 daily, TB-500 twice weekly | SubQ near injury site |
| Maintenance/Prevention Protocol | BPC-157: 250mcg 1x/day + TB-500: 2mg 1x/week | BPC-157 daily, TB-500 weekly | SubQ |
Reconstitution Instructions
- BPC-157 vial (5mg)
- TB-500 vial (2-5mg)
- Bacteriostatic water
- Insulin syringes (29-31 gauge)
- Alcohol swabs
- 1 Reconstitute each peptide separately
- 2 BPC-157: Add 2mL BAC water to 5mg vial = 250mcg per 0.1mL
- 3 TB-500: Add 2mL BAC water to 5mg vial = 250mcg per 0.1mL
- 4 Inject slowly along vial wall; swirl gently—never shake
- 5 Store reconstituted peptides refrigerated at 2-8°C
- 6 Do NOT mix peptides in same syringe unless pre-made blend
- 7 Use separate injection sites if administering same day
BPC-157 upregulates GH receptors; combined use may accelerate recovery.
GH secretagogues complement stack by increasing endogenous growth hormone.
MGF activates satellite cells; combined mechanisms may enhance muscle recovery.
Copper peptide promotes collagen; no negative interactions expected.
BPC-157 shows protective effects against NSAID-induced damage; long-term effects unknown.
Steroids may counteract healing; BPC-157 shown to reverse some steroid-induced damage in models.
Both peptides promote angiogenesis; monitor for bleeding issues.
Angiogenesis promotion could theoretically support tumor growth; contraindicated with cancer history.
Possible reduction in acute inflammation and pain at injury sites
Noticeable improvement in recovery between workouts; reduced soreness
Significant improvement in chronic injuries; improved joint comfort
Optimal healing effects; structural improvements in damaged tissues
Benefits may persist as healed tissue maintains integrity
Common Side Effects
- Not extensively documented in human studies
- Generally well-tolerated in available research
Stop Signs - Discontinue if:
- Any unusual lumps, growths, or rapid tissue changes
- Severe injection site reactions or infections
- Signs of allergic reaction (rash, swelling, difficulty breathing)
- Unexpected bleeding or bruising
- Persistent headaches or vision changes
- Any symptoms suggesting abnormal growth
Contraindications
- ANY history of cancer or suspicious growths
- Active malignancy or concurrent chemotherapy
- Pregnancy or breastfeeding
Good Signs
- White or off-white lyophilized powder cake indicating proper freeze-drying
- Crystal clear solution after reconstitution with bacteriostatic water
- Certificate of Analysis with HPLC purity testing (>98%) and mass spectrometry verification
Warning Signs
- Minor clumping acceptable if dissolves completely with gentle swirling
Bad Signs
- Collapsed, yellowed, or powder stuck to vial sides—may indicate degradation from heat
- Persistent cloudiness or visible particles after reconstitution—indicates contamination or degradation
- BPC-157 + TB4 Knee Pain Study (Lee & Padgett)(2021)
16 patients, intra-articular injection: Overall 87.5% (14/16) experienced knee pain relief. BPC-157 alone: 91.6% improvement (11/12). BPC-157 + TB4 combination: 75% improvement (3/4).
- BPC-157 Systematic Review (Vasireddi et al.)(2025)
36 articles reviewed. Preclinical models showed improvements in muscle tears, tendon ruptures, ligament tears, and fracture healing. Mechanism involves enhanced GH receptor expression and angiogenesis.
- Thymosin β4 Clinical Wound Healing (Treadwell et al.)(2012)
Two Phase 2 clinical trials showed thymosin β4 accelerated wound healing by approximately one month in responsive ulcer patients.
- Thymosin β4 Regenerative Properties Review (Goldstein et al.)(2012)
Comprehensive review establishing thymosin β4 as regenerative, promoting cell migration, reducing inflammation/apoptosis, and decreasing scar formation.
- BPC-157 Achilles Tendon Healing(2003)
Rats, 10 μg/kg i.p. daily, 14 days: BPC-157 demonstrated superior healing of transected Achilles tendons with improved biomechanical properties.
- Thymosin β4 Wound Healing Acceleration(1999)
Rats, full thickness wounds: Topical or intraperitoneal Tβ4 increased re-epithelialization by 42% at 4 days and up to 61% at 7 days.
Disclaimer
This information is for educational and research purposes only. Consult a healthcare professional before use.