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SS-31

Well Studied

Mitochondrial-Targeted Peptide | Cardiolipin Protector

Dose 5-10mg general support, 10-40mg clinical/performance protocols
Frequency Once daily (morning or pre-workout for athletic use)
Cycle 4-12 weeks for therapeutic protocols
Storage Reconstituted: 2-8°C in amber/dark vials, use within 30 days. Light sensitive

SS-31 (Elamipretide) is an aromatic-cationic tetrapeptide that selectively binds to cardiolipin in the inner mitochondrial membrane, preventing lipid peroxidation and optimizing electron transport chain function for enhanced cellular energy production.

Mechanism of Action

Selectively binds to cardiolipin in inner mitochondrial membrane, preventing lipid peroxidation and optimizing electron transport chain function. This improves ATP production and reduces oxidative damage at the mitochondrial level.

Key Benefits

  • Directly protects mitochondria
  • Improves cellular energy production (ATP)
  • Reduces oxidative damage
  • Enhances exercise capacity
  • Excellent safety profile in clinical trials
  • Cardioprotective effects
Type
Aromatic-cationic tetrapeptide

Mitochondrial

  • Enhanced ATP Production

    Optimizes electron transport chain function for improved cellular energy.

  • Oxidative Stress Reduction

    Prevents cardiolipin peroxidation and reduces mitochondrial oxidative damage.

  • Mitochondrial Disease Support

    Significant improvements in 6-minute walk test and fatigue scores in PMM patients.

Cardiovascular

  • Cardiac Protection

    Reduces cardiac injury markers and improves mitochondrial function in surgical patients.

  • Heart Failure Support

    Reduces ischemia-reperfusion injury and supports cardiac function.

  • Exercise Tolerance

    Enhanced exercise capacity and reduced fatigue.

Anti-Aging

  • Age-Related Muscle Function

    Reversed age-related decline in muscle function in animal studies.

  • Cognitive Protection

    Neuroprotective effects demonstrated in Alzheimer's models.

  • Cellular Rejuvenation

    Supports overall cellular health through mitochondrial optimization.

Subcutaneous or intravenous injection. SS-31 dissolves readily and should be protected from light due to photosensitivity.

GoalDoseFrequencyRoute
General mitochondrial support5-10mgOnce dailySubQ
Athletic performance10-20mgOnce daily pre-workoutSubQ
Clinical protocols40mgOnce dailySubQ or IV
Acute cardioprotection0.25mg/kg/hrContinuous infusionIV

Reconstitution Instructions

Materials Needed:
  • SS-31 peptide vial
  • Bacteriostatic water or saline
  • Insulin syringes (for SubQ)
  • Alcohol prep pads
  • Sterile filter (optional for IV)
  • Amber vials (light protection)
  1. 1 Clean vial with alcohol pad
  2. 2 Add 1-2mL bacteriostatic water per 10mg peptide
  3. 3 Gently swirl to dissolve (dissolves readily)
  4. 4 Verify clear, colorless solution
  5. 5 Optional: sterile filtration for IV use
  6. 6 Label with concentration and date
  7. 7 Store immediately at 2-8°C
  8. 8 Protect from light - use amber vials if available
NAD+

Both support mitochondrial function through different mechanisms.

synergistic
MOTS-c

Both are mitochondrial peptides with complementary mechanisms.

synergistic
Humanin

May work together to protect cellular function.

synergistic
GHK-Cu

Different mechanisms; no conflicts.

compatible
BPC-157

BPC-157 focuses on tissue repair while SS-31 targets cellular energetics.

compatible
Thymosin Beta-4

Both cardioprotective via different pathways.

compatible
Day 1-3

Subtle energy improvements, reduced fatigue

Week 1-2

Better exercise endurance, faster recovery

Week 2-4

Improved stamina, clearer thinking, better sleep

Week 4-8

Significant exercise capacity improvements

Week 8-12

Sustained energy, potential biomarker changes

Common Side Effects

  • Excellent safety profile in clinical trials
  • No significant side effects reported at therapeutic doses
  • Mild injection site reactions possible
  • Safe for long-term use based on current data

Stop Signs - Discontinue if:

  • Severe injection site reactions
  • Allergic reaction signs (rash, breathing difficulty)
  • Unusual fatigue or weakness (paradoxical reaction)
  • Cardiovascular symptoms
  • Severe headaches or vision changes

Contraindications

  • Known hypersensitivity to peptides
  • Pregnancy or breastfeeding (limited data)

Good Signs

  • Pharmaceutical grade (>98% purity required for clinical applications)
  • Clear, colorless solution after reconstitution
  • Certificate of Analysis available
  • Proper cold chain maintenance

Warning Signs

  • Light sensitive - protect from exposure using amber vials
  • Solution pH should be 6.5-7.5 (physiological range)

Bad Signs

  • Yellow or brown discoloration indicates degradation
  • Cloudy solution or visible particles
  • Improper storage conditions
  • Primary Mitochondrial Myopathy Phase 2 Trial
    (2018)

    40mg daily for 12 weeks in 36 patients showed significant improvements in 6-minute walk test and fatigue scores with excellent safety profile.

  • Cardioprotection in Heart Failure Study
    (2019)

    0.25mg/kg/hr infusion during cardiac surgery reduced cardiac injury markers and improved mitochondrial function.

  • Age-Related Muscle Dysfunction Study
    (2020)

    8 weeks in aged mice reversed age-related decline in muscle function, increased ATP production, and reduced oxidative stress.

  • Barth Syndrome Clinical Trial
    (2021)

    Phase 3 trial with 40mg daily for 48 weeks showed trends toward cardiac improvement in this rare mitochondrial disorder.

Disclaimer

This information is for educational and research purposes only. Consult a healthcare professional before use.